Neurology Seminar "Multicolor in vivo fluorescence imaging of Ca2+ and cAMP in the brain"

Event Details

Neurology Seminar will take place on November 12 at Kanazawa University Takara-machi ?Tsuruma campus (Takara-machi district).

Ca2+ and cAMP are important second messengers. In order to investigate the relationship between Ca2+ and cAMP in vivo, we have developed RCaMP3, a fluorescent protein whose red fluorescence brightens when it binds to Ca2+, and cAMPinG1, a fluorescent protein whose green fluorescence brightens when it binds to cAMP. Both RCaMP3 and cAMPinG1 were expressed in neurons in the cerebral cortex of living mice, and two-color simultaneous imaging using two-photon excitation microscopy revealed that the information expressed by firing was stored in the cells in tens of seconds during the process of transmission as Ca2+ and cAMP signals.

We will introduce the usefulness of such multicolor imaging, as well as the fluorescent sensors that have been actively developed around the world in recent years.

【Title】 Multicolor in vivo fluorescence imaging of Ca2+ and cAMP in the brain
Dr. Tatsushi Yokoyama from Institute for Medical and Biological Research, Kyoto University

【A course eligible for credit】
●Division of Medicine (Doctoral Course), Graduate School of Medical Sciences
Course name: "Up-to-date Seminar"

● School of Medicine
Course Title "Latest Medical Research"

[Location] Kanazawa University Takara-machi Campus Medical Library 2nd Floor Juzen Studio
　　　　　　http://square.umin.ac.jp/top/map/med-lib.pdf

Contact

Kanazawa University Faculty of Medicine Department of Neurology Hiroshi Kawasaki
E-mail: kawasaki-labo@umin.ac.jp